ASYMMETRICAL EFFECTS OF LESIONS IN FR2 ON LEFT-RIGHT RESPONSE DIFFERENTIATION IN THE RAT

Michael Noonan, Dennis Chmiel, Jr. and Seymour Axelrod

Canisius College and The State University of New York at Buffalo


Supported in part by NSF IBN-9209551.

Presented at Society for Neuroscience, Miami Beach, Florida, November, 1994.

 

INTRODUCTION

This study focused on the behavior of rats obliged sequentially to learn two different left-right associative learning tasks in the same maze. In an earlier study, Noonan and Axelrod (1992) examined the effects of complete left or right hemidecortication on such learning, and found two suggestions in the behavior of the rats of lateral asymmetry in the neural mediation of the learning. First, the rats demonstrated a consistent leftward turning bias, which was greater in strength on the second of the two tasks. Second, rats with cortical ablation on opposite sides of the brain showed dramatically different facility with second-task acquisition: rats with left hemidecortication acquired second tasks more slowly, and rats with right hemidecortication more quickly, than controls.

The present study investigated the consequences of cortical lesions confined to the left or right FR2 area (Zilles nomenclature) in an effort to learn if these asymmetric effects could be localized to a limited subdivision of the cortical hemisphere.

PROCEDURE

Long-Evans rats were prepared with either left- or right-FR2 ablation. Six weeks later the subjects were trained in sequential weeks on two different tasks in a water-filled M-maze (Fig. 1).

1. Brightness Discrimination (BD). On each trial, only one side of the maze was illuminated, the side varying randomly from trial to trial. The escape ramp was always located in the illuminated arm.

2. Left-Right Response Differentiation (LRRD). On each trial, both arms of the maze were either lit or unlit, this condition varying randomly from trial to trial. When the arms were illuminated, the escape ramp was always in the right arm. When the maze was unlit, the escape ramp was in the left arm.

On each task, testing continued for 25 trials per day until the rat made 10 successive correct responses.

RESULTS

Turning Bias. Turning bias was computed by tabulating direction of the first turn made on each trial and computing for each rat the ratio R/(L+R). On the first task (BD), our animals were about as often left-biased as right-biased, regardless of the side of lesion. On the second task (LRRD), both groups showed a left-turning bias, particularly the left-lesioned rats (Fig. 2).

Acquisition Scores. Trials-to-criterion was taken as the index of proficiency on each task. The left- and right-lesioned rats did not differ on the first (BD) task, but did differ on the second (LRRD) task (Fig. 3): rats with right-sided lesions learned the second task faster than rats with left-sided lesions.

DISCUSSION

The tendency of the rats to develop leftward behavioral biases on the second of these two tasks replicates and confirms earlier observations on total hemidecortication. It seems to imply an attempted right-hemisphere dominance under these circumstances.

That right-lesioned rats handle second learning tasks better than left-lesioned ones is also consistent with our earlier observations, and implies that the right hemisphere, when present, interferes in some way with the transition to the new associations of the second task. That this effect is seen when the cortical damage is limited to FR2 suggests that the relevant asymmetry is motor and/or attentional in nature.

Both findings indicate that there is asymmetrical involvement of the two sides of the rat brain in second-task learning, and that the direction (sidedness) of this asymmetry is consistent across the population.

Contact Info: Michael Noonan, PhD, Canisius College , 2001 Main St., Buffalo, NY 14208                                                                             noonan@canisius.edu